Table 1.
List of primary hits from phenotypic screen with podocytes
| Compound Name | Molecular Weight | Known Bioactivity | Z Score | Confirmation on Visual Check? |
|---|---|---|---|---|
| Agelasine | 458.1 | Cytotoxic, antineoplastic | −1.40 | Yes |
| Altrenogest | 310.4 | Progestin, antineoplastic | −1.44 | Yes |
| Antimycin A | 534.6 | Antifungal, depletes ATP, interferes with cytochrome oxidation | −1.77 | Yes |
| Apigenin | 270.2 | CK-II inhibitor, induces autophagy | −1.50 | Yes |
| Berberine chloride | 371.8 | Antiarrhythmic, α2-agonist, cholinesterase | −1.89 | Yes |
| Bupropion | 276.2 | Antidepressant | −1.63 | Yes |
| Chloroguanide.HCl | 290.2 | Antimalarial | −1.99 | Yes |
| Derrusnin | 356.3 | Mechanism of action unknown | −1.52 | Yes |
| Diosmetin | 300.3 | CYP1A1 inhibitor, antioxidant | −1.72 | Yes |
| Doxazosin mesylate | 547.6 | Antihypertensive | −1.53 | Yes |
| GSK 429286 | 432.4 | ROCK inhibitor | −2.94 | Yes |
| GSK3b inhibitor | 327.2 | GSK-3b inhibitor | −3.06 | Yes |
| Mundoserone | 342.4 | Natural product, rotenone analog, mechanism of action unknown | −1.72 | Yes |
| Mundulone acetate | 476.5 | Mechanism of action unknown | −1.28 | Yes |
| Norgestimate | 369.5 | Progestin | −1.32 | Yes |
| Papaverine hydrochloride | 375.9 | Muscle relaxant (smooth), cerebral vasodilator | −1.41 | Yes |
| Pyrintegrin | 451.5 | β1-Integrin agonist | −1.86 | Yes |
| Quinamide isopropylidene | 231.3 | Mechanism of action unknown | −1.59 | Yes |
| Rosolic acid | 290.3 | Diagnostic aid | −1.47 | Yes |
| Salicyl alcohol | 124.1 | Anesthetic (local), anti-inflammatory | −1.32 | Yes |
| SB 203580 | 377.4 | p38 MAPK inhibitor | −2.13 | Yes |
| Thiazovivin | 311.4 | ROCK inhibitor | −2.42 | Yes |
| Thioguanine | 167.2 | Antineoplastic, purine antimetabolite | −2.20 | Yes |
| Y27632 | 320.3 | ROCK inhibitor | −1.99 | Yes |
| Alsterpaullone | 293.3 | CDK inhibitor | −1.32 | No |
| Aurothioglucose | 392.2 | Antirheumatic | −1.73 | No |
| Cloxyquin | 179.6 | Antibacterial, antifungal | −1.35 | No |
| Cryptotanshinone | 296.4 | Angiogenesis inhibitor | −1.37 | No |
| Hinokitiol | 164.2 | Antifungal, mettaloprotease inhibitor, DNA synthesis inhibitor | −2.00 | No |
| Osajin | 404.5 | Antioxidant | −1.38 | No |
| Oxyquinoline hemisulfate | 243.2 | Anti-infectant | −1.34 | No |
| Quercetin tetramethylether | 358.4 | AD-R ligand, antibacterial | −1.86 | No |
| SB 202190 | 331.3 | p38 MAPK inhibitor | −1.47 | No |
| Trimethoprim | 290.3 | Antibacterial | −1.32 | No |
HCS assay–based identification of compounds that protect podocytes from injury. List of compounds identified as hits in the primary screen. The table shows compound name, its molecular weight, known biologic activity, and the Z score in the HCS assay. The last column shows whether visual inspection of the images of wells verified the compound as a hit in the primary screen. CK-II, casein kinase II; CYP1A1, cytochrome p450, family 1, subfamily A, polypeptide 1; ROCK, Rho-associated protein kinase; CDK, cyclin-dependent kinase; AD-R, adenosine receptor.