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. 2015 Oct 29;15:468. doi: 10.1186/s12879-015-1174-7

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© Vetter et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Detection of wildtype and mutated VLPs by diagnostic tests. VLPs were diluted to 10 IU/ml in negative human plasma and analysed with the indicated diagnostic tests. Panels a, b and d show antigen detection results of wildtype (wt) and mutated VLPs for those diagnostic tests which failed to detect wildtype VLPs harbouring the S16T (a and b) or K170R/N variant (d). Panels c and e show results of antigen detection by the Abbott Architect HIV Ag/Ab Combo to control for the presence of p24 antigen. Lines indicate S/Co = 1, which represents the lower limit of S/Co ratios for unequivocally positive samples according to the manufacturer’s instructions. In case of the bioMérieux VIDAS HIV p24 II the line indicates 3 pg/ml, denoting the lower limit of the quantifiable range for this test