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. 2015 Oct 29;15:481. doi: 10.1186/s12879-015-1212-5

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© Clancy et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — RPA assay robustness. The graphs show the fluorescence intensity obtained from the amplification of 50, 20 and 4 genome equivalents (GE) of S. pneumoniae (type strain; DSM20566), a in the absence of background human genomic DNA, b in the presence of 200 ng background human genomic DNA and c in the presence of 400 ng background human genomic DNA. Also shown in each graph is the fluorescence intensity obtained from the amplification of 100 GE (positive control, PC) of S. pneumoniae (type strain; DSM20566) and a no template control reaction (NTC)