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. 2015 Oct 29;15:481. doi: 10.1186/s12879-015-1212-5

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© Clancy et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Blood spiking. The graph shows the fluorescence intensity obtained from the RPA of 1 μL purified DNA (9.25, 0.925 and 0.0925 CFU equivalents) extracted from whole blood spiked with varying CFU of S. pneumoniae (type strain; DSM20566). Also shown is the fluorescence intensity obtained from the amplification of 100 GE (positive control, PC) of S. pneumoniae (type strain; DSM20566) and a no template control reaction (NTC)