Table 2.
Nucleic Acid sequences of primers and probes
| Probe/primer | DNA sequence (5’-3’) | Nucleotide positiona | Amplicon size (bp) |
|---|---|---|---|
| RPA | 190 | ||
| Forward | ACAGCTCCGTCTGTTATTTACAAAGTTAATTTGA*C | 1123–1157 | |
| Reverse | AGTCCCCACGCTTACGCTGAGCTAGCTCCATTAC*T | 1278–1312 | |
| Probe | CTTGACATAAGGCTCTTCAATGGTCGCAATCT[T(FAM)]A(dSpacer)[T(BHQ-1)]TGGGTCTGGAAAC*T | 1193–1242 | |
| PCR | 107 | ||
| Forward | CTCGTAAGCGTAAACTCCTTG | 1706–1727 | |
| Reverse | CATACTCAAGACGCTGAGGA | 1793–1813 | |
| Probe | FAM-ACGCATGAAATCCATCGGATCAGTT-TAMRA | 1749–1773 |
aThe nucleotide position refers to the LepA gene from S. pneumoniae strain SPNA45 (Genbank accession number NC_018594.1)
*indicates a phosphorothioate bond