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Env-ms, but not Env-syn, induces a dose-dependant production of IL-6 and IL-8. (A) Analysis of IL-6 production by HCMEC/D3 cells. Cells were treated overnight with TNF-α, Env-ms or Env-syn, then IL-6 was measured in the supernatants of the culture by ELISA. Results represent the mean ± SE of two independent experiments each performed in duplicate. (B) Analysis of IL-8 production by HCMEC/D3 cells. Same conditions as for IL-6 measurements. *P ≤ 0.05; **P ≤ 0.01.
