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. 2015 May 8;27(11):545–553. doi: 10.1093/intimm/dxv025

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© The Author 2015. Published by Oxford University Press on behalf of The Japanese Society for Immunology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 3. — Env-ms has significant, but different effects on HUVECs, compared to HCMEC/D3 cells. (A) Flow cytometry analysis of ICAM-1 expression after overnight treatment of HUVECs with TNF-α, Env-ms or Env-syn. (B) Analysis of IL-6 production by HUVECs. Cells were treated overnight with TNF-α, Env-ms or Env-syn, IL-6 was measured with ELISA. (C) Analysis of IL-8 production by HUVECs. Same conditions as for IL-6 measurements. Results represent the mean ± SE of 2–4 independent experiments each performed in triplicate. *P ≤ 0.05; **P ≤ 0.001.