Skip to main content
. 2015 May 8;27(11):545–553. doi: 10.1093/intimm/dxv025

Fig. 5.

Fig. 5.

Effects of Env-ms are greatly impaired after knock down of TLR4 receptor. (A) siRNA-transfected HCMEC/D3 cells were stimulated overnight with LPS or Env-ms. ICAM-1 expression was measured by flow cytometry. Cells were either not transfected with siRNA (NT) and not stimulated with LPS or Env-ms (untreated), or transfected with control siRNA (CT siRNA) or TLR4 siRNA and stimulated with Env-me (2µg ml−1) or LPS (0.1 µg ml−1). Results represent the mean ± SE of three independent experiments each performed in triplicate. (B) Same as in (A): untransfected cells (NT) or cells transfected with TLR4 siRNA were stimulated with TNFα (100U ml−1, 18h). (C, D) HCMEC/D3 cells were transfected and then stimulated overnight with LPS or Env-ms. Production of IL-6 and IL-8 was measured by ELISA assays. Results represent the mean ± SE of two independent experiments each performed in triplicate. NT: not transfected, CT: control. *P ≤ 0.01; **P ≤ 0.001.