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NP-LPS induces long-term and secondary antibody response in vivo. Three C57BL/6 mice (8-12 week old) were injected intraperitoneally with NP-LPS (25 μg) in PBS at d 0 and re-injected with NP-LPS (10 μg) in PBS at d 90 (second arrow). Sera were collected prior to the first injection and at different days, and analyzed for titers of NP3-binding IgG2b and IgG3 (depicted as relative units). Titers at d 0 were beyond the detection limit and set as 1 for the graph purpose.
