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. Author manuscript; available in PMC: 2016 Feb 1.
Published in final edited form as: Autoimmunity. 2015 Feb;48(1):1–12. doi: 10.3109/08916934.2014.993027

Figure 8.

Figure 8

CD154-induced B cell class switching and differentiation into PNAhi B cells and plasma cells are inhibited by CpG in the absence of BCR crosslinking (flow cytometry analysis). (a) CSR to IgG1 (top panels) and expression of PNA-binding lectins (bottom panels) in B cells stimulated with CD154 (3 unit/ml) plus IL-4 in the absence or presence of CpG (0.3 μM) for 4 d. (b) CSR to IgG1 (top panels) and expression of PNA-binding lectins (bottom panels) in B cells stimulated with CD154 (3 unit/ml) plus IFN-γ in the absence or presence of CpG for 4 d. (c) CSR to IgG1, IgE or IgG2a in B cells stimulated with CD154 plus IL-4 or IFN-γ in the absence or presence of CpG for 4 d. Data are mean and s.d. from three independent experiments (p values calculated by paired student t test). (d) Formation of B220loCD138hi plasma cells in cultures of B cells stimulated with LPS (1 μg/ml) plus IL-4 or IFN-γ in the absence or presence of CpG (0.3 μM) for 4 d. Data are mean and s.d. from three independent experiments (p values calculated by paired student t test).