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CpG-mediated inhibition of LPS- or CD154-induced CSR requires co-stimulation of B cells by CpG with LPS or CD154. (a) Levels of transcripts, as indicated, in B cells stimulated with CpG plus IL-4 for 0, 4 or 24 h (real-time qRT-PCR analysis). (b, c) CSR to IgG1 in CFSE-labeled B cells stimulated with LPS (3 μg/ml, b) or CD154 (3 unit/ml, c) plus CpG (0.3 mM) for the period as indicated plus IL4 (flow cytometry analysis). Data are representative of three independent experiments.
