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. Author manuscript; available in PMC: 2015 Oct 29.
Published in final edited form as: Mol Cell Neurosci. 2011 Aug 11;48(3):205–216. doi: 10.1016/j.mcn.2011.07.010

Figure 1. Expression of the dnTcf4 protein from the ROSA26 locus can be controlled by doxycycline.

Figure 1

A. In the presence of Doxycycline (Dox), the tetracycline-regulated transactivator (tTA) is unable to bind to the hCMV*-1 promoter (Tetracycline response element followed by the minimal promoter of the human cytomegalovirus immediate early gene) and initiate downstream gene expression.

B. Once the drug is removed from the tissue culture medium, the tTA binds the promoter and the dnTcf4 and Venus proteins are transcribed.

C. Control cells inducibly express Venus, but lack the dnTcf4 cDNA.