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. Author manuscript; available in PMC: 2015 Oct 29.
Published in final edited form as: Mol Cell Neurosci. 2011 Aug 11;48(3):205–216. doi: 10.1016/j.mcn.2011.07.010

Figure 6. Transfection of ESC with a plasmid containing a β-catenin shRNA decreases the level of β-catenin protein.

Figure 6

A. Diagrams of plasmids containing mutated shRNA control or β-catenin shRNA that were expressed in ESC and pregnant dams.

B. Western blot of protein from ESC transfected with shRNA constructs for 24 hours targeting β-catenin singly (lanes 2,3) or in combination (lane 4) versus mutated control shRNA constructs singly (lanes 5,6) or in combination (lane 7) demonstrating 40-64% knock-down. Top blot is β-catenin and bottom blot is a loading control blotted for β-actin. Lane 1 is protein from untransfected ESC.