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. 2015 Oct 29;13:77. doi: 10.1186/s12951-015-0141-1

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© Gallina et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Assessment of stemness and differentiation potential in cultured hMSCs. Immunophenotypic characterization of UT and SiO₂-NPs-treated hMSCs at t0 (a) and R 7d (b). An anti-isotype IgG was used as negative control for the reaction. c, d Representative images of UT (c) and SiO₂-NPs (d) cells after 18 days of adipose differentiation. Lipid vacuoles are stained with Oil Red. Magnification 10×, scale bar 100 µm. (e, f) Representative pictures of UT- (e) and SiO₂-NPs -exposed (f) hMSCs after 21 days of osteogenic differentiation. Alizarin Red was used to reveal extracellular deposition of calcium salts. Magnification 10×, scale bar 100 µm