Fig. 5.

HIF-2α-induced chemokine expression in chondrocytes causes FLS migration and invasion. a Migration and invasion of FLS treated (12 hours) with conditioned medium (CM) prepared from FLS infected with 800 MOI Ad-C or Ad-Epas1. Relative fold changes are presented (n = 6). b Migration and invasion of FLS treated (12 hours) with CM prepared from chondrocytes infected with 800 MOI Ad-C or Ad-Epas1. Relative fold changes are presented (n = 6). c Migration and invasion of FLS treated (12 hours) with CM prepared from WT (Epas1 ^+/+) or Epas1 ^+/− chondrocytes treated with IL-1β (2 ng/ml, 24 hours) (n = 4). d Migration of FLS treated with CM prepared from chondrocytes exposed to IL-1β (2 ng/ml, 24 hours) in the presence of IgG or neutralizing antibodies against CXCL2 and CCL5. Values presented as mean ± standard error of the mean (*P <0.05, **P <0.005). Scale bar: 50 μm. Ab antibody, CON control, FLS fibroblast-like synoviocytes, IL interleukin