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. 2015 Oct 29;10(10):e0141572. doi: 10.1371/journal.pone.0141572

Fig 1. L-citrulline protects C2C12 muscle myotubes from wasting.

Fig 1

Myotube diameter after 5 h incubation in HEPES buffered saline (HBS) with increasing concentrations of L-citrulline or isomolar concentrations of L-alanine (B) and representative images (20× objective) at the optimal dose of 2.5 mM (A). Myotube diameter for cells incubated in differentiation media (DM) or: HBS for 5 h (C) or; serum free (SF) media for 48 h (D). In each model, cells were treated with vehicle (PBS), 2.5 mM L-arginine or 2.5 mM L-citrulline (n = 5–8 per group). Values are means ± SE. For (B) comparisons were made using a two-way ANOVA (treatment × dose) with Fisher’s LSD post-hoc test. Significant differences are displayed where appropriate. For (C-D), Comparisons were made using a one-way ANOVA with Tukey’s post hoc test. Different letters denote significant differences (P<0.05) between groups, where a>b>c.