(a) Representative of nuclei (blue) and actin (green) in A549 cells treated with different pharmacological agents. The scale bar respresents 10 µm. (b) Average the nuclear aspect ratio as a function of the cell aspect ratio in control and nocodazole or Y27632 treated A549 cells on rectangular micropatterns with different aspect ratios. Data are shown as Mean ± SD with 8 ≤ n ≤ 15. ANOVA followed by Tukey's post tests yield the following p values: p=0.13 (ratio 2), p=0.029 (ratio 3), p= 0.0021 (ratio 4), p=0.0033 (ratio 5) for nocodazole; p=0.85 (ratio 2), p=0.75 (ratio 3), p= 0.56 (ratio 4), p=1.01×10−5 (ratio 5) for Y27632. Statistical significance is calculated with respect to shape ratio 1 in control, nocodazole, or Y27632 treated cells respectively. *p<0.05, **p<0.005.