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. 2015 Oct 29;10(10):e0141676. doi: 10.1371/journal.pone.0141676

Fig 4. Impact of antisense oligomers hybridized to the U180-A218 hairpin on FLp53 translation in MCF-7 cells under conditions of oxidative and genotoxic stress.

Fig 4

After oligomer transfection the cells were treated with (A) hydrogen peroxide at a final concentration of 100 μM and (B) 25 μM etoposide. Western blot analysis was performed with a monoclonal antibody (Pab 1801) to detect p53 levels. The GAPDH level was used as a loading control. The experiments were repeated at least twice. Representative Western blots are displayed in the figure. (C) The RT-PCR analysis of p53 mRNA and β-actin mRNA (as a control) extracted from the cells after 2′-OMe oligomers transfection and incubation the cells in the absence; c(-) and the presence (+) of hydrogen peroxide, respectively. At least two independent experiments were performed, which showed no changes in p53 mRNA level.