Fig 6. Impact of antisense oligomers on translation initiation from AUG1 for p53 and from AUG2 for Δ40p53, in rabbit reticulocyte lysates.

(A) Translation of the uncapped p53-554 RNA and (B) The uncapped Hp53-554 RNA constructs in the presence of specific oligomers No. 1, 4, 7, 7a, 7b and the control oligomer, respectively. The autoradiograms show the translation products from both initiation codons, AUG1 and AUG2, with p53-554 RNA (A) and Hp53-554 RNA (B). The bar graphs display translation efficiency for AUG1 and AUG2 codons with p53-554 RNA (A) and Hp53-554 RNA (B). All values are averages of at least three independent experiments and were normalized to the translation efficiency of product from AUG1 for p53. (*) p-values were calculated using Student’s t-test.