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. 2015 Nov 1;26(21):3719–3727. doi: 10.1091/mbc.E15-02-0085

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© 2015 Braunstein et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — (A) Cells expressing VCAM-1 and the indicated p97 isoform were treated with CAM741 alone or with Velcade as indicated. Cellular lysate content was evaluated directly (input) or subjected to a Rpt6 IP to evaluate proteasomal association (Rpn11) of VCAM-1 and p97. The asterisk represents a nonspecific band in the Rpt6 IP. (B) VCAM-1 and p97 expressing cells were treated with CAM741 or Velcade as indicated. Cellular lysates were fractionated into supernatant and pellet and VCAM-1, and proteasomal (PSMA1) content distribution was evaluated by IB. Sec61 immunoblot serves as a positive marker for ER membrane distribution.