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. 2015 Nov 1;26(21):3719–3727. doi: 10.1091/mbc.E15-02-0085

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© 2015 Braunstein et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — (A) Cells expressing p97 and insulin isoforms (as indicated) were treated with Velcade or left untreated as indicated. Preproinsulin and proinsulin migration is indicated, and GFP expression was evaluated to demonstrate equal transfection and loading of the gel. (B) N-glycosylation–bearing insulin was expressed in cells, and cellular content was resolved to discriminate between the indicated insulin isoforms. Velcade treatment and p97 expression were performed as in A. PNGaseF treatment was performed on cellular lysate, and PSMA1 content served as a loading control for cell lysates.