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. 2015 Nov 1;26(21):3768–3776. doi: 10.1091/mbc.E15-07-0531

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© 2015 Nagpal et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — CENP-C^166-324 and CENP-C^601-864 localize to interphase and mitotic centromeres, respectively. (A) Schematic showing CENP-C domains (red) and the constructs used, along with their ability to rescue CENP-C depletion and localization throughout the cell cycle. (B) Representative images showing wild-type CENP-C (using an anti–ggCENP-C antibody), GFP-CENP-C^601-864, GFP-CENP-C^166-324, and GFP-CENP-C^1-643 localization during interphase and metaphase. Kinetochores in CENP-C conditional-knockout cells expressing GFP constructs were visualized by CENP-T immunostaining at 36 h after addition of tetracycline. Bars, 10 μm. (C) Live-cell imaging of cells expressing GFP-CENP-C^166-324. (D) Growth curves for CENP-C–deficient cells expressing the indicated CENP-C constructs. Tetracycline was added at t = 0, and the number of live cells was counted. (E) Localization of CENP-C^∆325–643 and CENP-T in CENP-C–deficient cells expressing CENP-C^∆325–643. Bars, 10 μm.