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. 2015 Nov 1;26(21):3803–3815. doi: 10.1091/mbc.E15-06-0443

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© 2015 Grega-Larson et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — COBL localizes to the base of BB microvilli. (A) Endogenous COBL (green) and F-actin (magenta) labeling in a single villus from mouse small intestine. Boxed corners indicate zoom region, which highlights terminal web localization of COBL. (B) SIM projection of an induced Ls174T-W4 cell showing localization of endogenous COBL (green) and F-actin (magenta). Boxed corners indicate zoom region. (C, D) Line scans (n = 25) parallel to the microvillar axis show the distribution of endogenous COBL in mouse small intestine and Ls174T-W4 cells, respectively. Arrows highlight the peak localization (L_P, mean ± SE) along the normalized microvillar axis. Scale bars, 5 μm.