FIGURE 5:

COBL is required for microvillar growth. (A) Schematic of experimental design. (B) Images of scramble control or COBL KD cells scored for BB-positive cells. Arrowheads highlight BB-positive cells. Scale bar, 40 μm. (C) SIM projection images of scramble control cell, COBL KD cell without a BB, and COBL KD cell with a BB stained with phalloidin. Scale bars, 5 μm. (D) Percentage of BB-positive cells in KD and rescue experiments. Mean ± SEM from four separate experiments; number per condition is listed within bars (Student’s t test, *p < 0.05, **p ≤ 0.008, ***p ≤ 0.0004). (E) Quantification of microvillar length from scramble, COBL KD, and rescue cells with COBLr that were scored as BB-positive cells in D. Boxes represent the 25th and 75th percentiles around the median, and the whiskers represent the 5th and 95th percentiles; 30 cells/condition (Mann–Whitney U test, ***p < 0.0005).