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. 2015 Nov 1;26(21):3803–3815. doi: 10.1091/mbc.E15-06-0443

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© 2015 Grega-Larson et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Syndapin-2, similar to COBL, localizes to the base of BB microvilli. (A) Endogenous syndapin-2 localization (green) and F-actin signal (magenta) in a villus from human small intestine. Boxed corners indicate zoom region; arrowheads highlight syndapin-2 localization near the base of the BB. (B) SIM projection of endogenous syndapin-2 (green) in an Ls174T-W4 cell reveals enrichment at the base of the BB. Boxed corners indicate zoom region. Arrowheads highlight syndapin-2 localization at the base of the BB. (C, D) Line scans parallel to the microvillar axis (n = 25) show the distribution of endogenous syndapin-2 in human small intestine and Ls174T-W4 cells, respectively. Arrows highlight peak localization. (E) SIM projection of EGFP-COBL (red), endogenous syndapin-2 (blue), and F-actin (green) shows colocalization of EGFP-COBL and syndapin-2 at the base of the BB. Boxed corners in the red/blue channel overlay indicate the zoom region. (F) Time series montage of an Ls174T-W4 cell coexpressing EGFP-COBL and mCherry–syndapin-2 undergoing cell polarization and BB assembly. (G) Perimeter line-scan analysis of the cell shown in C at t = 0 and 196 min; black arrow in right-hand plot marks the position of the BB. (H) Intensity profile over time of a 2.5-μm² region of interest at the base of the forming BB in F. Scale bars, 5 μm.