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. 2015 Nov 1;26(21):3857–3866. doi: 10.1091/mbc.E15-07-0481

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© 2015 Moser et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Ccq1-Tpz1^TPP1 interaction contributes to optimal Ccq1 accumulation and is required for Ccq1 Thr93 phosphorylation and telomerase recruitment. (A and B) Effects of disrupting Ccq1-Tpz1^TPP1 interaction on telomere association for (A) Ccq1 or (B) Trt1^TERT, monitored by ChIP assays. Error bars represent SEM from at least three experimental replicates for Ccq1 ChIP and at least seven experimental replicates for Trt1^TERT ChIP. Statistical analysis of ChIP data by two-tailed Student’s t test is shown in Supplemental Table S4. Expression levels of FLAG-tagged Ccq1 or myc-tagged Trt1^TERT were monitored by Western blot analysis. Anti-Cdc2 blots served as loading control. (C) Ccq1 Thr93 phosphorylation, detected by phospho-(Ser/Thr) ATM/ATR substrate antibody (Moser et al., 2011), is abolished in Ccq1-Tpz1^TPP1–interaction disruption mutants. Asterisk (*) indicates hyperphosphorylated form of Ccq1.