Skip to main content
. 2015 Nov 1;26(21):3867–3878. doi: 10.1091/mbc.E15-06-0442

FIGURE 7:

FIGURE 7:

A SHIP2-Mena-PTP1B complex regulates EGFR-PTP1B interaction in MDA-MB231 cells. (A) Total Mena protein was immunoprecipitated from Rat2 fibroblast lysate and immunoblotted for SHIP2. (B) Quantification of Mena-EGFR PLA in wild-type MDA-MB231 cells starved or stimulated with 1 nM EGF for 60 s. Experiment performed 72 h posttransfection with 25 nM control (siControl) or SHIP2 (siSHIP2)–targeted SMARTPool siRNA. Results are shown as mean with 95% confidence intervals; >20 cells/condition. Asterisks indicate significant difference by ANOVA with Tukey multiple-comparison test (**p < 0.01). (C) Representative images of EGFR-PTP1B PLA in MDA-MB231 cell lines 72 h posttransfection with siControl or siSHIP2. (D) EGFR-PTP1B PLA in MDA-MB231 cell lines 72 h posttransfection with siControl (indicated by “–“ in siSHIP2 line) or siSHIP2. Cells stimulated ± 0.25 nM EGF for 60 s. Results are shown as mean with 95% confidence intervals; > 50 cells/condition. Asterisks indicate significant difference by nonparametric Kruskal–Wallis test and Dunn’s multiple-comparison test (**p < 0.05). See also Supplemental Figure S7.