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. 2015 Oct 29;10(10):e0141696. doi: 10.1371/journal.pone.0141696

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© 2015 Jamet et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 1 — (A) Workflow schematic of the mutational analysis for the β2 adrenergic receptor. First step was to reduce the primary sequence of the mouse β2AR (mβ2AR) to that of the non-trafficking odorant receptor M71. Then we asked what is the C-terminus minimal requirement for trafficking and activity by analyzing Ct truncations series as well as Ct full deletion. Furthermore we analyzed the effects of mutating a putative retention signal that might be revealed by a Ct deletion. We also compared the phenotype of mouse and human β2AR activity mutants to M71. Finally, we analyzed the behavior of M71/mβ2AR chimera. (B) The 2D topology of mouse β2AR was generated using the online software Topo2 (see Material & Methods). Plasma membrane is symbolized by two horizontal lines with the extracellular compartment on top and intracellular compartment on the bottom. Putative transmembrane domains are located in between those two lines. Different shapes and colors are used to symbolize the residues targeted for mutations. N-linked glycosylation sites are indicated in black boxes and their N in diamonds.