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. 2015 Oct 29;10(10):e0141196. doi: 10.1371/journal.pone.0141196

Fig 5. Loss of nuclear DNA by macrophages after in vitro infection with L. amazonensis or L. guyanensis.

Fig 5

Peritoneal macrophages of BALB/c (A) or C57BL/6 (B) mice infected or not with L. amazonensis or L. guyanensis. After the indicated time points cells were lysed with hypotonic lysis buffer containing PI and nuclei were analyzed by flow cytometry. Bars represent mean ± SD of three independent experiments. A typical experiment is shown in histograms after macrophage nuclei gating and analysis by FlowJo. Gating strategy of cell nuclei is shown in S1B and S1C Fig.