Table III.

Comparison of the OD values of P-gp, measured using western blot analysis, and the expression levels of Ras and p-ERK1/2, measured using immunocytochemistry, at different time-points.

Protein	Treatment	OD value
		24 h	48 h	72 h
P-gp	As2O3 (0.1 µM)	0.349±0.001	0.32±0.003	0.29±0.002
	As2O3 (0.5 µM)	0.30±0.002	0.28±0.002	0.16±0.001
	CsA (4 µg/ml)	0.16±0.004	0.16±0.001	0.15±0.002
	SGC7901/ADM	0.32±0.061	0.38±0.010	0.38±0.005
	SGC7901/S	0.14±0.003	0.14±0.003	0.14±0.002
Ras	As2O3 (0.1 µM)	0.091±0.004	0.084±0.001	0.080±0.002
	As2O3 (0.5 µM)	0.089±0.003	0.078±0.003	0.076±0.003
	As2O3 (0.75 µM)	0.081±0.004	0.073±0.002	0.073±0.002
	CsA (4 µg/ml)	0.091±0.003	0.093±0.003	0.084±0.005
	SGC7901/ADM	0.093±0.003	0.090±0.003	0.091±0.004
	SGC7901/S	0.068±0.003	0.071±0.005	0.066±0.008
p-ERK1/2	As2O3 (0.1µM)	0.099±0.009	0.086±0.002	0.083±0.004
	As2O3 (0.5 µM)	0.086±0.007	0.080±0.002	0.070±0.001
	As2O3 (0.75 µM)	0.08±0.004	0.078±0.005	0.067±0.002
	CsA (4 µg/ml)	0.098±0.009	0.11±0.018	0.11±0.025
	SGC7901/ADM	0.11±0.008	0.11±0.012	0.11±0.014
	SGC7901/S	0.10±0.016	0.090±0.010	0.10±0.027

Data are presented as the mean ± standard deviation of three experiments. As₂O₃, arsenic trioxide; CsA, cyclosporin A; P-gp, P-glycoprotein; p-ERK, phosphorylated extracellular signal-regulated kinase; OD, optical density.