Figure 1.

Cyclopamine inhibits adhesion, invasion and migration of U251 cells. (A) U251 cells were incubated for 24 h with various concentrations of cyclopamine. Cells were seeded into 96-well plates coated with fibronectin. After 1 h, the adhered cells were analyzed by MTT assay. The adhesion rate was expressed as a percentage of the control (0 µm). (B) U251 cells were seeded in the upper wells without matrigel coating and treated with various concentrations of cyclopamine. After 12 h, cells on the bottom side of the filter were fixed, stained and counted (magnification, ×400). The migration rate was expressed as a percentage of the control (0 µm). (C) U251 cells were seeded in the matrigel-coated upper wells and treated with various concentrations of cyclopamine. After 24 h, cells on the bottom side of the filter were fixed, stained and counted (magnification, ×400). The invasion rate was expressed as a percentage of the control (0 µm). Values are expressed as mean ± standard deviation of three independent experiments. ^*P<0.001, compared with controls.