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. 2015 Sep 29;12(5):7412–7418. doi: 10.3892/mmr.2015.4387

Fig. 5.

Fig. 5

Isoflurane induces p53 Ser15 phosphorylation and increases p53-dependent p21 transcriptional activity in WT NSCs, although not in NE-4C (p53−/−) cells. (A) Following the enrichment of the total p53 protein by immunoprecipitation, western blotting revealed a significant increase in the proportion of phospho-Ser15-p53 in the isoflurane-treated cells (**P<0.01; #P>0.05 compared with the non-treated group; n=3). (B) ChIP-polymerase chain reaction revealed that the binding efficiency of p53 to the p21 promoter at two independent p53 protein-binding sites was much higher following isoflurane treatment (**P<0.01; #P>0.05 compared with the non-treated group; n=3). ChIP, chromatin immunoprecipitation assay; IB, immunoblot; IgG, immunoglobulin G; p-p53, Ser15-phosphorylated p53; WT NSCs, wild-type neural stem cells.