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. 2015 Sep 14;12(5):6782–6788. doi: 10.3892/mmr.2015.4323

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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MUC1 gene silencing induces apoptotic phenotypic alterations in SMMC-7721 cells. (A) Detection of cell nuclear condensation by immunofluorescence with Hoechst 33342 staining. GFP was expressed stably in NC, MR1-C6 and MR1-D4 cells. (B) Apoptotic cells were analyzed by flow cytometry with Annexin V-PE staining. (C) Agarose gel electrophoresis of cellular genomic DNA fragmentation. DL2000 was the DNA marker. (D) Expression of caspase-3 and its activating products was determined by western blotting. (E) Expression of PARP and its cleaved products was determined by western blotting. MUC1, mucin 1; NC, negative control; PARP, poly (ADP ribose) polymerase; GFP, green fluorescent protein.