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. 2015 Sep 14;12(5):6782–6788. doi: 10.3892/mmr.2015.4323

Figure 3.

Figure 3

MUC1 gene silencing induces apoptosis of SMMC-7721 cells through mitochondrial and death receptor apoptotic pathways. (A) Mitochondrial extracts were assessed by western blotting to detect the levels of cytochrome c in the mitochondria. (B) Cytosol extracts were assessed by western blotting to detect the levels of cytoplasmic cytochrome c. (C) Western blotting for the expression of cleaved caspase-9. (D) Western blotting for the expression of caspase-8. Cox IV served as the mitochondrial loading control; β-actin was used as a protein loading control for whole cell lysis or cytoplasmic extracts. **P<0.01, compared with the NC. MUC1, mucin 1; NC, negative control.