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. 2015 Sep 14;12(5):6782–6788. doi: 10.3892/mmr.2015.4323

Figure 4.

Figure 4

MUC1 gene silencing decreases MUC1-CT binding with Bax and caspase-8 directly to induce apoptosis of SMMC-7721 cells. (A-C) Relative mRNA levels of Bcl-2, Bax and p53 (fold change) compared with the NC, *P<0.05, **P<0.01. (D) Western blotting for the expression of p53, Bcl-2 and Bax. (E) Cell lysates were subjected to immunoprecipitation with the anti-MUC1-CT antibody or normal IgG and then immunoblotted with anti-caspase-8 and anti-Bax antibodies. Whole cell lysate was not subjected to immunoprecipitation. MUC1, mucin 1; NC, negative control; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; CT, cytoplasmic tail; IgG, immunoglobulin G.