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. 2015 Oct 29;10(10):e0141312. doi: 10.1371/journal.pone.0141312

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Fig 1 — (A) After recombinant trimeric HA protein was coated onto 96-well plates, CT302 IgG₁ (■) or negative-control anti-respiratory syncytial virus IgG₁ (□) was incubated in wells as primary detection antibodies. Antibody bound to the recombinant His-tagged trimeric HA protein was detected with an HRP-conjugated anti-human IgG by addition of HRP substrate. Results represent the mean±S.D. obtained from duplicate wells per each condition. (B) Recombinant His-tagged trimeric HA proteins from H1, H3, and H5 strains were resolved by SDS-PAGE and transferred to nitrocellulose membranes, which were probed with CT302 IgG₁ or anti-His antibody. (C) Amino acid sequences in the V_H and V_L regions of CT302 IgG₁. HA, hemagglutinin; HRP, horseradish peroxidase.