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Surface observation of the (Aa) siRNA-free gel and the (Ab) gel loaded with siRNA using scanning electron microscopy and (Ac and d) fluorescence microscope, respectively. (B) Cumulative siRNA release profile under neutral and acidic conditions, determined using the RiboGreen assay. Data are expressed as the mean ± standard deviation. *P<0.05, compared with neutral. siRNA, small interfering RNA.
