Figure 3.

Phase-contrast microscopic images of K562 cells treated with Hyp-PDT (magnification, x400). The K562 cells were incubated with DMSO alone (control cells) or with 0.4 µg/ml Hyp (Hyp-PDT-reated cells) for 5 h; and exposed to irradiation for 4 min using an LED lamp at 0.3 mW/cm² light intensity. (A) Control cells prior to irradiation. (B) Hyp-PDT-treated K562 cells 8 h post-irradiation. (C) Hyp-PDT-treated K562 cells 16 h post-irradiation. (D) K562 cells cultured for 16 h without any treatment. The black arrows in indicate cells exhibiting severe deformation and cell shrinkage. PTD, photodynamic therapy; Hyp, hypericin.