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. 2015 Sep 15;12(5):6711–6717. doi: 10.3892/mmr.2015.4328

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Copyright: © Ren et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Effect of IMD on MC3T3-E1 cell differentiation. (A) Relative ALP activity. No differences were observed between the 100 nM IMD group and the control group at days 7 or 14 following culture in osteoblastic differentiation medium. (B) Relative expression levels of osteogenic markers on days 14 and 21. No differences were observed in the mRNA levels of OCN and BSP. (C) Alizarin red staining of mineralized MC3T3-E1 cells on days 14 and 21 following osteogenic differentiation. No significant effect was observed. Data are presented as the mean ± standard deviation. IMD, intermedin; ALP, alkaline phosphatase; OCN, osteocalcin; BSP, bone sialoprotein.