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BNP overexpression is followed by increased apoptotic activity in HCM cells. (A) Flow cytometric analysis of HCM cells treated with various concentrations of BNP, and stained with Annexin V-FITC and PI. (B) Western blots of caspase-1, -3, -7 and-8 in BNP-stimulated HCM cells. The protein expression levels were normalized to those of GAPDH. BNP, B-type-natriuretic peptide; FITC, fluorescein isothiocyanate; PI, propidium iodide.
