Figure 4.

lncRNA LSINCT5 knockdown by siRNA prevents BNP-induced apoptosis. (A) Cell proliferation was assessed by immunofluorescence under a confocal microscope at 24 and 72 h (magnification, x100). (B) The expression of caspase-1 and IL-1β was detected by western blot analysis, GAPDH was used as an internal control. (C) Effects of lncRNA LSINCT5 knockdown on BNP-induced HCM apoptosis determined by flow cytometry. The cells were stained with Annexin V-FITC and PI. lncRNA, long non-coding RNA; BNP, B-type-natriuretic peptide; siRNA, small interfering RNA; FITC, fluorescein isothiocyanate; PI, propidium iodide.