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. 2015 Sep 9;12(5):6517–6526. doi: 10.3892/mmr.2015.4300

Figure 4.

Figure 4

The expression of VEGF, CD34 and FVIII/Rag proteins in fibrosarcoma. (A) The immunohistochemical staining of the expression of VEGF, CD34 and FVIII/Rag proteins in the wt, Hsf1-/- and mHsf1 fibrosarcoma tissues. The images were taken using a 40X objective. (B) Quantification of the expression of VEGF in the fibrosarcoma tissue with the AIOD method. The quantification of the expression of (C) CD34 and (D) FVIII/Rag in the Hsf1-/- fibrosarcoma using the MVD method. Data are presented as the mean ± standard deviation, one-way analysis of variance. **P<0.01, ***P<0.001 vs. wt and mHsf1 cells. VEGF, vascular endothelial growth factor; CD34, cluster of differentiation 34; FVIII/Rag, factor VIII related antigen; wt, wild type; Hsf1, heat shock factor 1; mHsf1, Hsf1 null MEF cells expressing mouse Hsf1. AIOD, average integral optical density.