Figure 4.

OE of Eaf2 induces apoptosis. (A) Cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling assay after transfection with Eaf2 OE vector (scale bar, 100 µm). (B) Western blot analysis revealed the effects of Eaf2 OE on cleaved caspase-3 and cleaved caspase-9 protein levels. (C) The protein levels of bax and bcl-2 were detected by western blotting after transfection with Eaf2 using β-actin as reference. Values are expressed as the mean ± standard deviation of three experiments. ^**P<0.01 compared with the vector. bcl-2, B-call lymphoma 2; bax, bcl-2-associated X protein; OE, overexpression; Eaf2, ELL associated factor 2.