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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Lab Invest. 2015 Aug 17;95(11):1278–1290. doi: 10.1038/labinvest.2015.104

Figure 2.

Figure 2

EZH2 expression in human cultured RB cells versus retina and RPE. (A, B) Western blot of human RB cell lines WERI-Rb1 and Y79, primary human fetal RPE cultures, and whole human retina isolated from midgestational and adult stages showed that EZH2 protein levels were highest in RB tumor cells, followed by fetal week (fw) 19 retina, and lowest in primary human fetal RPE cultures and adult retina. β-actin was used as a loading control. (C) Hematoxylin & eosin (H&E) and corresponding (D, E) EZH2 staining of fw19 human retina. (E) Black arrowhead indicates nuclear EZH2 staining of ONBL retinal progenitor cells while white arrowhead shows lack of nuclear EZH2 staining in RPE cells. RPE cells contain brown melanosome granules in their cytoplasm. Original magnification ×400 (C, D), ×600 (E). (F) Immunofluorescence of cultured WERI-Rb1 cells showed co-expression of EZH2 (red) and CRX (green) in all nuclei (DAPI, blue). Ret retina; GCL ganglion cell layer; ONBL outer neuroblastic layer; RPE retinal pigment epithelium.