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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Arthritis Rheumatol. 2015 Nov;67(11):2933–2943. doi: 10.1002/art.39291

Figure 1.

Figure 1

Candidate osteoarthritis DAMPs induce MCP-1 production by DRG cells primarily through TLR4. A) Wild-type DRG cells were stimulated with 0, 0.5, or 1 μg/mL S100A8 ± the small molecule TLR4 inhibitor Tak242 (Tak, 1 μM) or with 10 ng/mL IL-1α. Representative of three independent experiments. B) Wild-type DRG cells were stimulated with 0, 50, or 100 μg/mL α2-macroglobulin, ± Tak242 (Tak, 1 μM). Representative of three independent experiments. C) Fold-change plot combines data from the three independent experiments for S100A8 and α2-macroglobulin (α2m); **p<0.01 vs no stimulation. D) Wild-type DRG cells were stimulated with 1 μg/mL LPS ± 1 μM Tak242. Representative of two independent experiments. E) Tlr4−/− DRG cells were stimulated with 1 μg/mL S100A8, 100 μg/mL α2-macroglobulin, or 10 ng/mL IL-1α. Representative of three independent experiments for S100A8 and two experiments for α2-macroglobulin. F) DRG cells taken from mice 8 weeks after surgery were incubated ± 1 μM Tak242. Representative of three independent experiments. *p<0.05, **p<0.01, ***p<0.001; mean±SEM.