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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Arthritis Rheumatol. 2015 Nov;67(11):2990–3003. doi: 10.1002/art.39247

Figure 2. Primary APS neutrophils demonstrate enhanced NET release.

Figure 2

A, Freshly-isolated neutrophils from healthy controls or APS patients (n=18) were seeded onto coverslips and incubated in serum-free media for 2 hours. NET release was scored by immunofluorescence microscopy. B, Representative immunofluorescence microscopy of control (top) and APS (bottom) neutrophils as presented in panel A. Blue=DNA and green=neutrophil elastase. Scale bars=25 microns. C, Control neutrophils were treated with 10% sera from heterologous healthy controls or APS patients for 2 hours. NET release was scored by immunofluorescence microscopy. *p<0.05, ***p<0.001, and ****p<0.0001; ns=not significant. In addition to individual data points, mean and standard deviation are also presented.