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. Author manuscript; available in PMC: 2016 Nov 21.
Published in final edited form as: Chem Commun (Camb). 2015 Nov 21;51(90):16176–16179. doi: 10.1039/c5cc05744b

Fig. 1. Tethering DLPs to functionalized surfaces.

Fig. 1

(a) Schematic to illustrate the immunocapture procedure used to tether asynchronously transcribing RV DLPs to antibody (IgG)-decorated surfaces via protein A adaptors. (b) Transcribing DLPs tethered to EM grids or SiN microchips in the presence of VP6-specific IgGs showed varying lengths of associated mRNA transcripts (white arrows) in cryo-EM images. (c) DLPs prepared in the absence of nucleotides needed for transcription do not show associated mRNA in cryo-EM images. (e) EM specimens prepared in the absence of IgGs generally failed to recruit DLPs. Scale bar is 100 nm.