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. 2015 Oct 29;10(10):e0141712. doi: 10.1371/journal.pone.0141712

Fig 2. M71::GFP does not traffic to the plasma membrane.

Fig 2

(A) M71::GFP expression in OP6 cells in trapped perinuclearly (A’) Same cell with colabelling of plasma membrane revealed by CellMask Red. (B) mβ2AR::GFP expression in OP6 cells gives a flat morphology that has sharp focus. Expression is observed in filopodia. (B’) Same cell as (B) with colabelling of plasma membrane revealed by CellMask Red. (C, D) M71::GFP and mβ2AR::GFP expression vectors. (E) Filopodia counts by Cell Mask Red and GFP for mβ2AR::GFP and mβ2AR::GFP. (F) M71::GFP co-expression with mβ2AR::mCherry in OP6 cells maintains GFP fluorescence trapped perinuclearly whereas (F’) mβ2AR::mCherry expression is found in the filopodia.