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. 2015 Sep 15;7(9):1528–1540.

Figure 4.

Figure 4

PX-12 potentiates anti-tumor effect of 5-FU in vitro. A. 2000 cells/well were plated overnight followed by treatment with increasing doses of PX-12and 5-FU in combination at a fixed ratio (PX-12/5-FU=1.25). CCK8 assay was used to calculate the inhibition rate of PX-12 or 5-FU on cells. B. CI was obtained using CalcuSyn2.0 for synergy analyses. C. Annexin V/PI staining was used to identify apoptosis induced by PX-12 and/or 5-FU. D. The percentage of cell apoptosis was shown as the mean ± SEM from three independent experiments. E. Western blot analysis of the expression levels of apoptosis-related protein in HepG2 cells which incubated for 48 h. All the results are represented as the mean ± SEM from three independent trials. *P<0.05, **P<0.01.