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. 2015 Sep 15;7(9):1541–1552.

Figure 4.

Figure 4

NF-κB activation protects SH-EP1 cells from NO-induced apoptosis. A. SH-EP1 and I-κBαM cells were treated with IGF-1 (100 ng/ml) for 0.5, 1, and 2 h. NF-κB activation was determined using NF-κB reporter assay. Data are from three independent experiments and expressed as mean ± S.E. *P < 0.05 and **P < 0.01, vs IGF-1 treated SH-EP1 cells. B. SH-EP1 cells and I-κBαM cells were treated with IGF-1. Phospho-I-κBα and I-κBα in SH-EP1 cells and I-κBαM cells were determined by Western blot assay. β-actin (ACTB) served as a loading control. C. SH-EP1 cells and I-κBαM cells were incubated with SNP (1 mM) in the presence or absence of IGF-1 for 24 h. Data are expressed as mean ± S.E. from at least three experiments performed in triplicate. *P < 0.05, vs SH-EP1 cells.